While on vacation in Romania, Josh, the main character, and his wife Kate are on a train with several other tourists. During the train ride, Kate is about to tell Josh something when she discovers he was hiding a bottle of alcohol, hinting at a drinking problem. Angry, she walks out and heads to the dining car. Josh comes across a Romanian man who tells him that his wife left a letter for him, and then Josh reads it. Feeling guilty, Josh heads to the dining car to apologize. On his way, he encounters many travellers, such as a student,two ballerinas called Sacha and Tasha, four teenagers Monica, Max, Aaron and Katja, are going to a rave, an English psychiatrist called Jane, two Romanian generals, a Romanian ticket collector, a German businessman and Yeli, a fortune-teller who gives Josh a grim prediction. He then proceeds further only to find the car covered in blood. Bursting into the car, he find his wife being carried out by a large man with an odd-looking mechanism on his face. One of the generals tries to stop the man, but is ripped in half. Josh and Yeli follow and catches a glimpse of a strange man who is laughing manically. Suddenly, the train derails. Josh awakens to find the train in a river, and tries to save the ticket collector, and fails. The general calls him to shore, and Josh runs to a cave with other survivors in it.

The businessman walks away from the cave into the forest and is killed by something off screen. Panicking, the survivors run deeper in the cave until they fall out the other side into a swamp. While making their way out the other general is pulled under and killed, causing the survivors to panic. After escaping the swamp and walking through the forest, they come upon a cemetery where they meet up with another group of survivors. The student wanders into a cellar and is heard screaming. Josh runs to help and finds her panicking, saying she saw a corpse move. Josh investigates and the corpse, along with several others reanimate and attack. The girl runs away and Josh fends them off before pursuing her. Josh exits the cellar and finds that all the survivors have run off due to the zombies. As Josh makes his way through the cemetery, he hears a scream and watches as Monica is decapitated and then thrown at a wall, the attacker being Ernst, his wife's kidnapper. Ernst then subdues Josh, who loses consciousness.

Josh awakens to find that he has been tied to a chair by Viktor, the mad scientist responsible for the zombie infestation. Max is tied up as well, but is soon killed by Viktor. He is about to kill Josh when he gets a phone call and exits urgently, leaving a zombie nurse to finish the job. Fortunately, Josh is rescued and freed by the Romanian who told Josh of Kate's letter, and they attempt to find their way out. The two make their way though a hall when the survivor inspects a fresh bloodstain, only to end up bisected by a trap. Josh fights his way through a horde of zombies and encounters Jane, but she is kidnapped by Ernst. He later comes across a ringing phone. Josh answers and hears Kate begging him to help, followed by a woman's voice telling him that he will never find her,and a projector shows footage of a webcam with a mysterious woman next to Kate, who is captive in a torture chair. He continues to find strange bedrooms, of which is a crib and a doll making sounds of crying. He then finds a sewing room, filled with dresses and mannequins one of which strangely looks like a zombie, casting a bit of paranoia to the scene. He advances to a cave to find the two Russian ballerinas now zombies. The strange woman from before traps him in with the zombie twins. After he defeats them, he wanders outside to a courtyard.